rabbit monoclonal anti phospho pka substrate rrxs t antibody (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc rabbit monoclonal anti phospho pka substrate rrxs t antibody
Rabbit Monoclonal Anti Phospho Pka Substrate Rrxs T Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phospho+pka+substrate/bio_rxiv__64898__2026__03__29__715116-323-8-14
Average 86 stars, based on 1 article reviews

rabbit monoclonal anti phospho pka substrate rrxs t antibody - by Bioz Stars, 2026-10

86/100 stars

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Incubation:

Article Title: ANKRD53 is downregulated in human obesity and coordinates lipolysis with mitochondrial oxidative metabolism in adipocytes.
Article Snippet: Proteins in the lysates were separated by SDS-PAGE and 170 transferred onto nitrocellulose membranes (Cytiva, 10600001). .. Membranes were 171 blocked with 5% non-fat milk in TBST and incubated overnight at 4 °C with the 172 following primary antibodies: ANKRD53 (Proteintech, 24283-1-AP), Tubulin (Sigma-173 Aldrich, T6199), phospho-HSL (Ser660) (Cell Signaling Technology, 45804), HSL 174 (Cell Signaling Technology, 4107), Phospho-PKA Substrate (Cell Signaling 175 Technology, 9624), and Histone H3 (Cell Signaling Technology, 4499). .. After washing, 176 membranes were incubated for 1 h at room temperature with HRP-conjugated 177 secondary antibodies (Cell Signaling Technology,7074 for anti-rabbit IgG, 7076 for 178 anti-mouse IgG).

Article Title: Glucagon promotes net hepatic glycogen repletion following meal ingestion.
Article Snippet: Protein concentration of lysates was determined with a Pierce BCA assay (Thermo Fisher). .. Membranes were blocked in EveryBlot (BioRad) and incubated in phospho-PKA substrate (Cell Signaling Technology; 9624), phospho-glycogen synthase (serine 641; Cell Signaling Technology; 4703), or HSP90 (Cell Signaling Technology; 4877). .. Bands were detected by the ChemiDoc Imaging System (Bio-Rad) and band density was quantified using the Bio-Rad Image Lab. ProcartaPlexä AKT Assay.

Article Title: KLF7 induced ADRB3-dependent IL-6 production in brown adipocytes during stress
Article Snippet: Protein samples were separated by SDS‒PAGE and transferred to PVDF membranes (Millipore, P005505). .. The membranes were soaked in protein-free fast blocking buffer (Servicebio, G2052, China) for 10 min and then incubated overnight at 4°C with primary antibodies against KLF7 (Abcam, ab197690), IL-6 (Abcam, ab290735), phospho-PKA substrate (CST, 9624), p-CREB (Proteintech, 28792-1-AP, China), CREB (Proteintech, 12208-1-AP, China), β-Tubulin (ZSGB-BIO, TA-10, China), and UCP1 (Abcam, ab234430), followed by a 2-h incubation with goat anti-rabbit immunoglobulin G (IgG) (ZSGB-BIO, ZB-2301, China) and goat anti-mouse IgG (ZSGB-BIO, ZB-2305, China) secondary antibodies at room temperature. .. Finally, imaging was performed after incubating the membranes in chemiluminescent solution (Millipore, P90720 ).

Article Title: β Cell Gαs signaling is critical for physiological and pharmacological enhancement of insulin secretion
Article Snippet: Protein was quantified with a BCA assay (Thermo Fisher Scientific), and SDS-PAGE was performed. .. Membranes were blocked in 4% BSA in TBST and then incubated in phospho-PKA substrate (Cell Signaling; 9624). .. Immunoblots were developed with ECL substrate (Bio-Rad) and imaged in a ChemiDoc imager (Bio-Rad).

Western Blot:

Article Title: Partial truncation of the C-terminal domain of PTCH1 in cancer promotes tumourigenesis by non-canonical activation of a GLI-PI3K loop.
Article Snippet: Signal intensity was quantified by ImageJ software and normalized to loading control indicated in the text. .. The following antibodies were used: EGFR (Cell Signaling Technology #2256, WB 1:1,000), AKT (Cell Signaling Technology #4691, WB 1:1,000), phospho- AKT (Ser473) (D9E) XP (Cell Signaling Technology #5012, WB 1:1,000), p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology #4695, WB 1:1,000), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Oncogene Signaling Technology #4376, WB 1:1,000), Gli1 (Cell Signaling Technology #2643, WB 1:1,000), Phospho-PKA substrate (Cell Signaling Technology #9624, WB 1:1,000), GAPDH-HRP (Proteintech #HRP-60004, WB 1:3,000), Vinculin (Santa Cruz #sc-73614, WB 1:10,000), Poly-ADP ribose Polymerase (Cell Signaling Technology #9542, WB 1:2,000), anti VP16 (Santa Cruz #sc7545, WB 1:1,000),HA-Tag (Invitrogen #26183, WB 1:10,000), goat antirabbit IgG HRP (BioRad #172-1019, WB 1:3,000), goat anti-mouse IgG (H L)HRP conjugate (Bethyl #A90-116P, WB 1:3,000). ..

Article Title: Partial truncation of the C-terminal domain of PTCH1 in cancer promotes tumourigenesis by non-canonical activation of a GLI-PI3K loop
Article Snippet: Signal intensity was quantified by ImageJ software and normalized to loading control indicated in the text. .. The following antibodies were used: EGFR (Cell Signaling Technology #2256, WB 1:1,000), AKT (Cell Signaling Technology #4691, WB 1:1,000), phospho- AKT (Ser473) (D9E) XP (Cell Signaling Technology #5012, WB 1:1,000), p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology #4695, WB 1:1,000), phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (Cell Signaling Technology #4376, WB 1:1,000), Gli1 (Cell Signaling Technology #2643, WB 1:1,000), Phospho-PKA substrate (Cell Signaling Technology #9624, WB 1:1,000), GAPDH-HRP (Proteintech #HRP-60004, WB 1:3,000), Vinculin (Santa Cruz #sc-73614, WB 1:10,000), Poly-ADP ribose Polymerase (Cell Signaling Technology #9542, WB 1:2,000), anti VP16 (Santa Cruz #sc7545, WB 1:1,000),HA-Tag (Invitrogen #26183, WB 1:10,000), goat anti-rabbit IgG HRP (BioRad #172-1019, WB 1:3,000), goat anti-mouse IgG (H L)-HRP conjugate (Bethyl #A90-116P, WB 1:3,000). ..

Article Title: p21-activated kinase 4 suppresses fatty acid β-oxidation and ketogenesis by phosphorylating NCoR1
Article Snippet: .. Antibodies used for Western blotting, immunofluorescence and immunohistochemical analyses Antibody Catalogue No. Dilution Fold Company Western Blot PAK4 (G222) 62690 1:2500 Cell Signaling Technology p-PAK4 (S474) 3241 1:2500 Cell Signaling Technology HSP90 (C45G5) 4877 1:2500 Cell Signaling Technology Ubiquitin (E4I2J) 43124 1:2500 Cell Signaling Technology HMGCS2 (D3U1A) 20940 1:2500 Cell Signaling Technology Phospho-PKA Substrate (100G7E) 9624 1:2500 Cell Signaling Technology Phospho-(Ser/Thr) 9631 1:2500 Cell Signaling Technology Sirt1 2310 1:2500 Cell Signaling Technology Sirt6 (D8D12) 12486 1:2500 Cell Signaling Technology Phospho-Akt (Ser473) (D9E) 4060 1:2500 Cell Signaling Technology Akt 9272 1:2500 Cell Signaling Technology Acetylated-Lysine 9441 1:2500 Cell Signaling Technology CHOP (L63F7) 2895 1:2500 Cell Signaling Technology Phospho-S6 Ribosomal Protein (Ser240/244) 2215 1:2500 Cell Signaling Technology S6 Ribosomal Protein (5G10) 2217 1:2500 Cell Signaling Technology NCoR1 5948 1:2500 Cell Signaling Technology NEDD4 2740 1:2500 Cell Signaling Technology ATF-6 (D4Z8V) 65880 1:2500 Cell Signaling Technology Phospho-PERK (Thr980) (16F8) 3179 1:2500 Cell Signaling Technology PAK4 (B-3) SC-390507 1:2500 Santa Cruz Biotechnology FGF21 (H-105) SC-292879 1:2500 Santa Cruz Biotechnology PPARα (H-2) SC-398394 1:2500 Santa Cruz Biotechnology Sirt4 3224 1:2500 Bioworld Technology LaminB1 (L75) BS3547 1:2500 Bioworld Technology GAPDH (A531) AP0066 1:2500 Bioworld Technology Sirt5 (aa30-46) LS-B2060 1:2500 LifeSpan Biosciences Sirt7 ( aa317-366) LS-B1566 1:2500 LifeSpan Biosciences FoxO1 (Acetyl-Lys294) LS-C800723 1:2500 LifeSpan Biosciences Sirt2 (EPR20411-105) ab211033 1:2500 Abcam Sirt3 ab189860 1:2500 Abcam GRP78 BiP ab21685 1:2500 Abcam NCOR2/SMRT ab24551 1:2500 Abcam CPT1A (8F6AE9) ab128568 1:2500 Abcam T-OXPHOS ab110413 1:2500 Abcam THRβ ab53170 1:2500 Abcam p-IRE1α ab48187 1:2500 Abcam MDM2 (2A10) ab16895 1:2500 Abcam LXRα 14351-1-AP 1:2500 Proteintech Proximity Ligation Assay NCoR1 5948 1:100 Cell Signaling Technology p300 (D8Z4E) 86377 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Immunofluorescence NCoR1 5948 1:100 Cell Signaling Technology PPARα (H-2) SC-398394 1:100 Santa Cruz Biotechnology Supplementary Table 4. ..

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rabbit monoclonal anti phospho pka substrate rrxs t antibody (Cell Signaling Technology Inc)

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Immunofluorescence:

Immunohistochemical staining:

Ubiquitin Proteomics:

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